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| Registros recuperados : 23 | |
| 4. |  | BRAGA, J. V.; FACCIN, J. C.; ZANI, G. da S.; MONDADORI, R. G.; LUCIA JÚNIOR, T.; MARQUES, M. G. Does Phenazine Ethosulfate improve early embryo development? In: INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTION, 9., 2022, Bento Gonçalves. Abstracts...Belo Horizonte: CBRA, 2022. Animal Reproduction, v. 19, n. 4, 2022.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | PIVATO, I.; PEREIRA, D. C.; PEIXER, M. A. S.; LUCIA JUNIOR, T.; DESCHAMPS, J. C.; RUMPF, R. Influência de diferentes tratamentos hormonais em doadoras de ovócitos na produção de embriões bovinos in vitro. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 5., 2000, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2000. p. 97.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | CORRÊA, E. K.; LUCIA JÚNIOR, T.; GIL-TURNES, C.; CORRÊA, M. N.; BIANCHI, I.; COREZZOLA, J. L.; ULGUIM, R. R. Efeito de diferentes profundidades de cama sobre parâmetros ambientais para suínos em crescimento e terminação. Revista Brasileira de Engenharia Agrícola e Ambiental, v.12, n.5, p.540-545, set./out., 2008.| Biblioteca(s): Embrapa Algodão. |
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| 7. | | DESCHAMPS, J. C.; LUCIA JUNIOR, T.; CORREA, M. N.; MACEDO JUNIOR, M. C.; RHEINGANTZ, M. G. T. Otimizacao da eficiencia do processo de producao animal a partir do uso de biotecnicas reprodutivas. Revista Brasileira de Reproducao Animal, Belo Horizonte, v.24, n.1, p.21-29, 2000.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | SILVA, J. F. da; MION, B.; MADEIRA, E. M.; VIEIRA, A. D.; LUCIA JUNIOR, T.; PEGORARO, L. M. C. Teste de toxicidade de uma solução de vitrificação de embriões bovinos (PIV) contendo EGMME E DMSO. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 23.; ENCONTRO DE PÓS-GRADUAÇÃO, 16., 2014, Pelotas. [Anais.]. Pelotas: UFPel, 2014.| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | PRADIEÉ, J.; CARDOSO, T. F.; SILVA, E. F.; LAZZARI, J. C.; GONÇALVES, A. O.; MONDADORI, R. G.; PEGORARO, L. M. C.; LUCIA JUNIOR, T. Antioxidants on crioula breend ram semen cryopreservation. Animal Reproduction, v. 9. n. 3. p. 558, jul/set, 2012. Publicado no: Proceedings of the 26th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), August 30th to September 2nd, 2012, Foz do Iguaçu, PR, Brazil.| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | PRADIEÉ, J.; VIANNA, L. L.; GONÇALVES, A. O.; SANTOS, E. C. da S.; MONDADORI, R. G.; LUCIA JUNIOR, T.; VIEIRA, A. D.; PEGORARO, L. M. C. B-mercaptoetanol na maturação e cultivo in vitro de embriões ovinos. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. Anais... Cumbuco: SBTE, 2011. p. 411.| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | VARGAS JÚNIOR, S. F.; COSTA, V. G. G.; OLIVEIRA, F. C. de; GOULARTE, K. L.; HAAS, C. S.; GASPERIN, B. G.; LUCIA JÚNIOR, T. Bovine sperm cell motility after incubation in follicular fluid; Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 7, p. 866-869, July, 2018. Scientific Notes.
Título em português: Motilidade de espermatozoides bovinos após incubação em fluído folicular.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | FERREIRA, C. E. R.; HAAS, C. S.; GOULARTE, K. L.; ROVANI, M. T.; CARDOSO, F. F.; SCHNEIDER, A.; GASPERIN, B. G.; LUCIA JUNIOR, T. Expression of paraoxonase types 1, 2 and 3 in reproductive tissues and activity of paraoxonase type 1 in the serum and seminal plasma of bulls. Andrologia, v. 50, n. 3, e12923, Apr. 2018.| Biblioteca(s): Embrapa Pecuária Sul. |
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| 13. | | SANTOS, E. C. da S.; PRADIEÉ, J.; GONÇALVES, A. O.; MONDADORI, R. G.; LUCIA JUNIOR, T.; VIEIRA, A. D.; PEGORARO, L. M. C. Novo meio para utilização do Brilliant Cresyl Blue na seleção de oócitos para produção in vitro de embriões suínos: dados preliminares. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. Anais... Cumbuco: SBTE, 2011. p. 427.| Biblioteca(s): Embrapa Clima Temperado. |
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| 14. | | GÜTHS, M. F.; SIQUEIRA, H. A.; MONTES, J. H.; MOREIRA, F.; RIZZOTO, G.; PERIPOLLI, V.; TUTIDA, Y. H.; LUCIA JUNIOR, T.; IRGANG, R.; KICH, J. D.; BIANCHI, I. Removal or substitution of in feed antimicrobials in swine production. Preventive Veterinary Medicine, n. 205, ed. 105696, 2022.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 15. | | SANTOS, ELISA C. S.; PRADIEÉ, J.; MADEIRA, E. M.; PEREIRA, M. M.; MION, B.; MONDADORI, R. G.; VIEIRA, A. D.; PEGORARO, L. M. C.; LUCIA JUNIOR, T. Selection of porcine oocytes in vitro through brilliant cresyl blue staining in distinct incubation media. Zygote, v. 25, n. 1, p. 49-55, Feb. 2017.| Biblioteca(s): Embrapa Clima Temperado. |
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| 16. | | MADEIRA, E.; MION, B.; SILVA, J. F. da; TORRES, F. C.; RINCON, J. A. A.; VEIRA, A. D.; PEGORARO, L. M. C.; LUCIA JUNIOR, T. Vitrificação de embriões bovinos (PIV) com etileno glicol monometil éter (EGMME): um novo crioprotetor. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Biblioteca(s): Embrapa Clima Temperado. |
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| 17. | | PRADIEÉ, J.; CARDOSO, T. F.; SILVA, E. F.; GONÇALVES, A. O.; GASTAL, G. D. A.; ROSA, C. E.; MONDADORI, R. G.; PEGORARO, L. M. C.; VIEIRA, A. D.; LUCIA JUNIOR, T. Effect of ß-Mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 68, n. 5, p. 1309-1315, 2016.| Biblioteca(s): Embrapa Clima Temperado. |
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| 18. | | SANTOS, E. C. da S.; MADEIRA, E. M.; MION, B.; PEREIRA, M. M.; SILVA, J. F. da; PRADIEÉ, J.; MONDADORI, R. G.; VIEIRA, A. D.; PEGORARO, L. M. C.; LUCIA JUNIOR, T. Brilliant cresyl blue staining and incubation media can affect the cortical granules migration of swine oocytes. The Journal Reproduction and Development, v. 59, Suppl, p. 137, 2013.| Biblioteca(s): Embrapa Clima Temperado. |
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| 19. | | SANTOS, E. C. S.; PRADIEÉ, J.; MADEIRA, E. M.; MION, B.; SCHIAVON, R. S.; PEREIRA, M. M.; MONDADORI, R. G.; VIEIRA, A. D.; PEGORARO, L. M. C.; LUCIA JUNIOR, T. Different media for selection of swine oocytes with Brilliant Cresyl Blue ? effects on parthenogenetic embryo development. Animal Reproduction, v.10, n.3, p. 564, Jul./Sept. 2013 Publicado no: roceedings of the 27th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), August 29th to September 1st, 2013, Praia do Forte, BA, Brazil.| Biblioteca(s): Embrapa Clima Temperado. |
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| 20. | | FERREIRA, C. E. R.; CAMPOS, G. S.; SCHMIDT, P. I.; SOLLERO, B. P.; GOULARTE, K. L.; CORCINI, C. D.; GASPERIN, B. G.; LUCIA JUNIOR, T.; BOLIGON, A. A.; CARDOSO, F. F. Genome-wide association and genomic prediction for scrotal circumference in Hereford and Braford bulls. Theriogenology, v. 172, p. 268-280, Sept. 2021.| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 23 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Rondônia. |
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Data corrente: |
14/06/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MORAES, F. P. de; D'AVILA, C. A.; OLIVEIRA, F. C. de; CASTRO, N. A. de; VIEIRA, A. D.; SCHNEIDER, A.; PFEIFER, L. F. M.; PEGORARO, L. M. C.; FERREIRA, R.; FERST, J. G.; ROVANI, M. T.; CORREA, M. N.; GONÇALVES, P. B. D.; LUCIA JUNIOR, T.; GASPERIN, B. G. |
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Afiliação: |
FABIANE PEREIRA DE MORAES, Federal University of Pelotas; CAMILA AMARAL D'AVILA, Federal University of Pelotas; FERNANDO CAETANO DE OLIVEIRA, Federal University of Pelotas; NATALIA ÁVILA DE CASTRO, Federal University of Pelotas; ARNALDO DINIZ VIEIRA, Federal University of Pelotas; AUGUSTO SCHNEIDER, Federal University of Pelotas; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; ROGERIO FERREIRA, Santa Catarina State University; JULIANA GERMANO FERST, Federal University of Santa Maria; MONIQUE TOMAZELE ROVANI, Federal University of Rio Grande do Sul; MARCIO NUNES CORREA, Federal University of Pelotas; PAULO BAYARD DIAS GONÇALVES, Federal University of Santa Maria / Federal University of Pampa; THOMAZ LUCIA JUINOR, Federal University of Pelotas; BERNARDO GARZIERA GASPERIN, Federal University of Pelotas. |
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Título: |
Prostaglandin F2a regulation and function during ovulation and luteinization in cows. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, v. 171, p. 30-37, Sept. 2021. |
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ISSN: |
0093-691X |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2021.05.008 |
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Idioma: |
Inglês |
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Conteúdo: |
Although prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF group (P < 0.05), and no effect of the treatments on intrafollicular P4 and E2 concentrations 24 h after GnRH. The prostaglandin metabolite (PGFM) concentrations in the FF were greater in cows receiving systemic PGF (P < 0.001), which prompted us to further check its role on ovulation. Therefore, in Experiment 3, in a final attempt to demonstrate the local effect of PGF on ovulation, cows with preovulatory follicles received an intrafollicular injection (IFI) of PBS (Control) or 100 ng/mL purified PGF (PGF group). PGF treatment did not affect the time of ovulation after IFI (66 ± 6.4 and 63 ± 8.5 h for control and PGF, respectively; P > 0.05), further suggesting that it has no direct effect in the ovulatory process. Based on our findings, we concluded that FM decreased PGF synthesis within the follicle, whereas PGF treatment decreased follicular vascularization. In addition, the in vivo model of intrafollicular injection evidenced that PGF alone is not able to locally induce ovulation. MenosAlthough prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF ... Mostrar Tudo |
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Palavras-Chave: |
Ação anti-inflamatória; F2a; Luteinização. |
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Thesagro: |
Ovulação; Prostaglandina; Reprodução Animal; Vaca. |
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Thesaurus NAL: |
Animal reproduction; Anti-inflammatory activity; Cows; Luteinization; Ovulation; Prostaglandins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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100 1 $aMORAES, F. P. de
245 $aProstaglandin F2a regulation and function during ovulation and luteinization in cows.$h[electronic resource]
260 $c2021
520 $aAlthough prostaglandins are important in the ovulation process, a precise role for prostaglandin F2a (PGF) has not been elucidated. This study aimed to evaluate the regulation of PGF receptor mRNA (PTGFR) in granulosa cells and the local effect of PGF on ovulation and luteinization. In Experiment 1, using samples collected in vivo before (Day 2), during (Day 3) and after (Day 4) follicular deviation, expression of PTGFR in bovine granulosa cells was more abundant in the dominant follicle after deviation than in subordinates (P < 0.05). However, the expression of PTGFR was not regulated (P ¼ 0.1) in preovulatory follicles at different time-points (0, 3, 6, 12 and 24 h) after ovulation induction with GnRH. In Experiment 2, to assess the role of systemic PGF treatment on luteinization and vascularization of preovulatory follicles, flunixin meglumine (FM), a nonsteroidal anti-inflammatory drug, was used to inhibit endogenous prostaglandin synthesis. Cows with preovulatory follicles were induced to ovulate with GnRH (0 h) and allocated to three groups: Control, with no further treatment; FM, treated with 2.2 mg/kg FM im 17 h after GnRH treatment; and FM þ PGF, treated with FM 17 h after GnRH, followed by 25 mg dinoprost tromethamine (PGF) 23 h after GnRH treatment. FM injection was able to reduce the concentration of PGF in the follicular fluid (FF) (P < 0.001). However, contrary to our hypothesis, color Doppler ultrasound evaluations revealed decreased vascular flow in FM þ PGF group (P < 0.05), and no effect of the treatments on intrafollicular P4 and E2 concentrations 24 h after GnRH. The prostaglandin metabolite (PGFM) concentrations in the FF were greater in cows receiving systemic PGF (P < 0.001), which prompted us to further check its role on ovulation. Therefore, in Experiment 3, in a final attempt to demonstrate the local effect of PGF on ovulation, cows with preovulatory follicles received an intrafollicular injection (IFI) of PBS (Control) or 100 ng/mL purified PGF (PGF group). PGF treatment did not affect the time of ovulation after IFI (66 ± 6.4 and 63 ± 8.5 h for control and PGF, respectively; P > 0.05), further suggesting that it has no direct effect in the ovulatory process. Based on our findings, we concluded that FM decreased PGF synthesis within the follicle, whereas PGF treatment decreased follicular vascularization. In addition, the in vivo model of intrafollicular injection evidenced that PGF alone is not able to locally induce ovulation.
650 $aAnimal reproduction
650 $aAnti-inflammatory activity
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650 $aLuteinization
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773 $tTheriogenology$gv. 171, p. 30-37, Sept. 2021.
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